We developed TetTCR-Seq, a platform for high-throughput pairing of TCR sequences with potentially multiple pMHC species bound on single T cells.
Library of fluorescently labeled, DNA-barcoded pMHC tetramers is constructed using in vitro transcription & translation.
Tetramer-stained cells are single-cell sorted to amplify the DNA-barcoded (with molecular identifier) and TCRαβ genes in RT-PCR.
Nucleotide-based cell barcodes are used to link multiple peptide specificities with their bound TCRαβ sequences.
Check out what you can do with this technology!
Track progeny of antigen-binding T cells in disease settings
Identify cross-reactivity and autoimmune events
Discover and validate immune response biomarkers
Enigneer and optimize neoantigen-specific T cells